For the last ten years, the process of extracting Platelet-Rich Plasma (PRP), while seemingly straightforward, has been a subject of ongoing controversy regarding the reliability of its methods. The utilization of a proper kit is crucial for the successful creation of PRP.
Although it is feasible to collect blood into a test tube and subject it to centrifugation, more than merely labeling it as PRP is required.
This version, often called “bloody PRP,” might yield about 1.5 times the quantity of platelets found in blood, if fortunate. Still, it will also contain many red and white blood cells.
This ineffective type of PRP can potentially lead to post-injection complications, making its use problematic.
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How to select the best PRP kit?
Let us begin by addressing the approach of not utilizing a kit. It involves drawing blood into a test tube, subjecting it to centrifugation, and then collecting the upper portion, often labeled as PRP.
However, this variant is known as “bloody PRP,” it proves to be the least effective form, containing merely 1 to 1.5 times the platelet count if luck is on your side.
Regrettably, instances have arisen where this “bloody PRP” method has led to post-injection flare-ups, underscoring its unsuitability for use.
Conversely, a Platelet-Rich Plasma kit yields a platelet concentration ranging from 5 to 7 times higher than the average blood level.
This concentrated serum, typically achieving a 5X to 7X elevation, is acknowledged as Platelet-Rich Plasma or Autologous Conditioned Plasma.
Professionally designed kits allow you to include or exclude red and white blood cells in the final PRP product, a choice tailored based on specific applications.
Therefore, it is better to know how different kits available may differ.
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1. Gel separators
Gel separators consist of a test tube containing a gel layer at the bottom. This gel serves to separate blood from platelets through osmosis. Nevertheless, a significant proportion of platelets also adhere to the gel in this process.
Consequently, the outcome typically increases the maximum platelet concentration by 1.5 times. However, a positive aspect is that this method effectively removes white and red blood cells, offering an advantage.
2. Buffy coat
Typically, kits revealing a visible buffy coat yield 5 to 7 times higher platelet concentrations, mainly platelets and white blood cells, with plasma above and packed blood below.
Ensuring separation of the buffy coat from red blood cells, free from contamination, achieves PRP containing under 10% red blood cells.
3. Buffy coat with a double spin
The ultimate type employs a red blood cell-free buffy coat. This top-tier kit involves spinning PRP twice to eliminate more red blood cells and enhance platelet concentration. The final step is isolating the buffy coat, resulting in pure PRP.
Amidst numerous PRP kits, the Biosafe kit stands out as the premier choice. Its superiority lies in complete control over the final product’s composition and attributes.
